Using vitellin monoclonal antibodies to assess the vitellogenesis-inhibiting hormone activity of Macrobrachium rosenbergii
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چکیده
منابع مشابه
Production of monoclonal antibodies specific to Macrobrachium rosenbergii nodavirus using recombinant capsid protein.
The gene encoding the capsid protein of Macrobrachium rosenbergii nodavirus (MrNV) was cloned into pGEX-6P-1 expression vector and then transformed into the Escherichia coli strain BL21. After induction, capsid protein-glutathione-S-transferase (GST-MrNV; 64 kDa) was produced. The recombinant protein was separated using SDS-PAGE, excised from the gel, electro-eluted and then used for immunizati...
متن کاملMonoclonal antibodies against extra small virus show that it co-localizes with Macrobrachium rosenbergii nodavirus.
The capsid protein (CP) gene of extra small virus (XSV) expressed in Escherichia coli as a 42 kDa glutathione S-transferase (GST)-fusion protein (GST-XCP) or a 20 kDa His6-fusion protein (His6-XCP) were purified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), combined, and used to immunize Swiss mice to produce monoclonal antibodies (MAbs). Using dot blot, Western blot,...
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In the present investigation, vitellogenesis during ovarian development in the freshwater prawn Macrobrachium rosenbergii was assessed. Ovarian development was classified into five stages based on size, colour and texture of ovary. Interestingly, histological results clearly indicate that the oocyte development gradually increased from stages I to V based on the yolk material accumulation. Besi...
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BACKGROUND Tributyltin (TBT) is a ubiquitous persistent xenobiotic that can be found in freshwater, estuarine and marine ecosystem. TBT is a strong endocrine disrupting compound (EDC) that can cause toxic threat to aquatic organisms. Imposex, sexual deformities and endocrine dysfunctions are the causes of TBT to most of the aquatic organisms. Effect of TBT on the vitellogenesis and sex hormonal...
متن کاملTolerance of Macrobrachium rosenbergii to white spot syndrome virus
The susceptibility of Macrobrachium idella, M. lamerrae and M. rosenbergii to white spot Ž . syndrome virus WSSV was tested by immersion challenge, oral route and intramuscular injection. Their susceptibility to WSSV was compared with that of Penaeus indicus and P. monodon. The WSSV caused 43.3% and 53.3% mortality in M. lamerrae and M. idella, respectively, by immersion method and 53.3% and 66...
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ژورنال
عنوان ژورنال: ScienceAsia
سال: 2014
ISSN: 1513-1874
DOI: 10.2306/scienceasia1513-1874.2014.40.157